Three-dimensional histological electrophoresis enables fast automatic distinguishment of cancer margins and lymph node metastases
Menée à l'aide de pièces de résection et de xénogreffes de tumeurs mammaires sur des modèles murins, cette étude met en évidence l'intérêt d'un système d'électrophorèse histologique tridimensionnelle pour évaluer avec précision les contours d'une tumeur et distinguer rapidement les régions tissulaires envahies des ganglions lymphatiques sentinelles
Tissue diagnosis is important during surgical excision of solid tumors for margin evaluation. Conventional histopathologic methods rely heavily on image-based visual diagnosis by specialized pathologists, which can be time-consuming and subjective. We report a three-dimensional (3D) histological electrophoresis system for rapid labeling and separation of the proteins within tissue sections, providing a more precise assessment of tumor-positive margin in surgically resected tissues. The 3D histological electrophoresis system uses a tumor-seeking dye labeling strategy to visualize the distribution of tumor-specific proteins within sections and a tumor finder that automatically predicts the tumor contour. We successfully demonstrated the system’s capability to predict the tumor contours from five murine xenograft models and distinguish the tumor-invaded region of sentinel lymph nodes. Specifically, we used the system to accurately assess tumor-positive margins from 14 patients with cancer. Our 3D histological electrophoresis system serves as an intraoperative tissue assessment technology for more accurate and automatic pathologic diagnosis. Next-generation intraoperative tissue-assessment technology enables more accurate and automatic pathologic diagnosis.
Science Advances , article en libre accès, 2022