Resveratrol protects mouse embryonic stem cells from ionizing radiation by accelerating recovery from DNA strand breakage
Menée in vitro, cette étude montre que le resvératrol, un polyphénol, protège les cellules souches embryonnaires de souris contre les rayonnements ionisants en accélérant la réparation de l'ADN
Resveratrol has elicited many provocative anti-cancer effects in laboratory animals and cultured cells, including reduced levels of oxidative DNA damage, inhibition of tumor initiation and progression, and induction of apoptosis in tumor cells. Use of resveratrol as a cancer-preventive agent in humans will require that its anti-cancer effects not be accompanied by damage to normal tissue stem or progenitor cells. In mouse embryonic stem cells (mESC) or early mouse embryos exposed to ethanol, resveratrol has been shown to suppress apoptosis and promote survival. However, in cells exposed to genotoxic stress, survival may come at the expense of genome stability. To learn whether resveratrol can protect stem cells from DNA damage and to study its effects on genomic integrity, we exposed mESC pre-treated with resveratrol to ionizing radiation (IR). 48-hour pre-treatment with a comparatively low concentration of resveratrol (10 μM) improved survival of mESC more than 2-fold after exposure to 5 Gy of X-rays. Cells pre-treated with resveratrol sustained the same levels of reactive oxygen species and DNA strand breakage after IR as mock-treated controls, but repaired DNA damage more rapidly and resumed cell division sooner. Frequencies of IR-induced mutation at a chromosomal reporter locus were not increased in cells pre-treated with resveratrol as compared to controls, indicating that resveratrol can improve viability in mESC after DNA damage without compromising genomic integrity.
http://carcin.oxfordjournals.org/content/early/2011/11/04/carcin.bgr236.abstract 2011